Projectes i publicacions / Projects and publications
Index
Projectes / Projects
| programme |
reference |
keywords |
description |
participants |
start |
end |
budget |
info |
||
|---|---|---|---|---|---|---|---|---|---|---|
| Portivity |
Portable Interactivity | FP6-IST | 027098 | IT Sector and European Commission
continuously recognise the importance of audio-visual services for the
Information Society, notably when such services make use of synergy
from broadcast and broadband networks and apply open, international
standards which, in turn, ensure horizontal markets. The porTiVity project will follow this line and develop a converged Rich Media iTV system which integrates broadcast and mobile broadband delivery to portables and mobiles, and which will enable the end-user to act on moving objects within video programmes or contents. porTiVity will further the technical achievements from SAMBITS (Rich Media iTV supported by MHP), SAVANT (quasi-synchronism of scaled Internet and broadcast content) and GMF4iTV (interactivity at the level of moving video objects).porTiVity will realise direct interactivity with moving objects on handheld receivers, which are connected to DVB-H or DMB as the mobile broadcast and to UMTS as the mobile communication channel. porTiVity will develop tools which enable producers to link Rich Media information with video objects. The project will further develop the playout for portable Rich Media iTV and the middleware, data and presentation engine in the handheld receiver. For the application layer, the project will make use of international standards such as MPEG-4 A/V, MPEG-7 Metadata, MPEG-21 DIA and MHP. On the distribution side, MXF is used as material exchange format. GMF4iTV has shown that interaction at object level is highly attractive to the consumer. porTiVity will widen the scope to handhelds which are, in contrast to TV-sets, personal devices (at "hand distance"), and thus ideally suited for interactivity. The project combines the necessary expertise of broadcasters, play-out and server-specialists, network operators, middleware developers as well as handheld terminal manufacturers across Europe. The consortium will contribute to the further development of international standards, notably DAB and DVB. |
|
01/01/2006 | 31/12/2008 | 2.722.808 € / 4.948.731 € | ||
| SUIT |
Scalable, ultra-fast and Interoperable Interactive television | FP6-IST | 028042 | The Internet has pushed the television
to a new era, that of interactivity. However, the main difference
between them is the amount of bandwidth required by the second to
transport TV/Video signals with good QoS. Mixing the internet protocol
and a broadcasting network (BN) may not result in an acceptable
interactive system. Moreover, BNs are unable to serve mobile users
moving at high speed. SUIT will explore several techniques to minimize
the above mentioned problems and will propose solutions in the context
of the universal interactive television access and network convergence.
SUIT will consider an end-to-end chain composed of a playout, last mile
networks, and terminals of different computational and display
capacities. Firstly, video contents, either live or pre-recorded will be encoded in a scalable way to be delivered simultaneously to a variety of devices from HD flat displays to handheld devices. Secondly, as the viewer expects that the interactive system responds to a request quickly, SUIT will optimally manage at the playout, the DVB-T/H and WiMAX resources, thereby, the requested content will be delivered as fast as possible through the available TDMA/FDMA slots in both networks. As SUIT is using scalable video contents, the playout system is able to increase, for short time, the bandwidth to support high priority contents, e.g. hyperlinked interactive video contents. Thirdly, scalable multiple descriptions, possibly unbalanced, are delivered through DVB-T/H and WiMAX in order to support high speed mobility. To achieve the objectives related to scalable video, SUIT will make use of the most recent technologies like scalable MPEG-4 AVC/H.264 and MPEG-21 DIA. The former will allow encoding digital video contents in a scalable manner. In order to optimally meet the network conditions, joint source-channel-modulation schemes will be investigated. Finally, the latter will support terminal descriptions and negotiations between the terminal and the playout. |
|
01/02/2006 |
30/04/2008 |
2.800.000 € / 4.515.547 € | ||
| Tarifa | The Atomic Redesign of the Internet Future Architecture |
i2Cat | Current Internet architecture has
remained nearly invariant since its inception in early 70’s. In
incorporating all the services and applications evolution made in
almost 40 years, the current architecture has become more and more
complex and ossified. Applications, services, networks and protocols
are evolving very quickly and it has been widely proved that TCP/IP is
not able to efficiently cover the emerging demands of applications,
mainly due to its rigid layered model.TARIFA proposes a new clean slate
network architecture to deploy ubiquitous services avoiding
hierarchical layering. TARIFA follows a service-oriented approach for a
flow-oriented context-aware network architecture where communications
are composed in situ using reusable and indivisible components called
Atomic Services and according to the needs and requirements of the
requester. |
|
02/2010 | 06/2011 | 354.088 € | |||
| Trilogy | fuTuRe Internet technoLOGY |
i2Cat |
TRILOGY is a multidisciplinary research project aimed at five specific thematic areas: Smart Optical Networks, Audiovisual Live Streaming Peer-to-Peer,
Internet of things, High Quality Video Systems and Infrastructure as a
Service and Virtualization. The objectives set in this project have a
dual approach: on the one hand, the scientific research results such as
scientific articles and patents; on the other hand, development results
closer to innovation such as demonstrations and prototypes. The work realized in the project resulted in a series of articles published on several outreach activities. |
|
10/2007 | 03/2009 | 536.414 € | |||
| RAFFI | Recerca d'Eines Antifrau Financer per Internet |
Acció (Generalitat de Catalunya) |
The objective of RAFFI is to conceive
new algorithms and tools to prevent, detect and correct possible
attacks the different channels of e-banking may be subjected to (WWW,
mobile, DVB-T). This project will allow real-time evaluation of the behavior of an access to any of the channels as well as real-time fraud detection based on the users’ behavior history when accessing these channels. |
01/2009 | 10/2010 | |||||
| Raudos | Red Interactiva Multiplataforma de Contenidos Audiovisuales |
|
TSI-020302-2008-115 |
The objective of RAUDOS is the design
and development of a Content Management System for interactive content
associated to a television show. A user of RAUDOS logins to the
platform to access a series of entertaining applications (e.g. quiz
games) related to the television program. These activities allow the
user to increase its virtual salary, which is used to buy additional
content (e.g. next episode previous). The project regards the access to
the platform from multiple devices (PC, mobile phones, connected TVs
and even information kiosks). |
|
01/2008 |
04/2009 |
|||
| Transgame |
Transmedia Games |
Ministerio de Industria, Turismo y Comercio (MITYC) - Plan Avanza 2 I+D | Transmission system of videogames on
hybrid interactive TV platforms, combining games that come from
broadcast and broadband channels according to user needs and available
technology. The technology developed for this project is based on the
HbbTV standard, extending some modules to adapt this standard to
TRANSGAME project needs. |
|||||||
| Phidias |
Plataforma Híbrida de Difusión Inteligente de Aplicaciones y Servicios para Televisión |
Ministerio de Industria, Turismo y Comercio (MITYC) - Plan Avanza 2 I+D Competitividad 2010 | PHIDIAS’s objective is to study and develop a complete hybrid television broadcasting platform
(HbbTV), which combines the Broadcast signal and TV through the
Internet. To this end, different alternatives are being researched
insofar as technological solutions for HbbTV are concerned, developing
specific applications (social networks, personalised publicity,
interactive applications). Lastly, a validation will be carried out
with a final demonstrator of the solution, both for ground and for
satellite television. |
|
2010 |
2011 |
||||
| i3Media | Tecnologías para la creación y gestión automatizada de contenidos audiovisuales inteligentes | CDTI CENIT Ingenio 2010 (tractor) |
PR.068-2007 | The main objective of i3media is to
generate a framework for the representation and manipulation of
intelligent digital contents and for the development of technologies
which enable a higher automatization of the audio-visual production.
In particular, the increase with respect to the state of the art will
involve the automatic association of the intelligence and
auto-adaptation functions with the content itself, independently of the
underlying communication networks. |
|
01/01/2007 |
31/12/2010 |
35M€ |
|
|
| programme |
reference |
keywords |
description |
participants |
start |
end |
budget | info |
Publicacions / Publications
| Title |
Authors |
pdf |
Abstract |
Publication |
Date |
Venue |
Research Gate |
Google Scholar |
|---|---|---|---|---|---|---|---|---|
| New Framework and Mechanisms of Context-Aware Service Composition in the Future Internet |
Gonzalez, A. J., Martin de Pozuelo, R., German, M., Alcober, J., Pinyol, F., |
The ongoing proliferation
of new services, applications, and contents is leading the Internet to
an architectural crisis owing to its inability to provide efficient
solutions to new requirements. Clean-slate architectures for the future
Internet offer a new approach to tackle current and future challenges.
This proposal introduces a novel clean-slate architecture in which the
TCP/IP protocol stack is decoupled in basic functionalities, that is,
atomic services (ASs). A negotiation protocol, which enables
context-aware service discovery for providing adapted communications,
is also specified. Then, we present how ASs can be discovered and
composed according to requesters’ requirements. In addition, a media
service provisioning use case shows the benefits of our framework.
Finally, a proof-of-concept implementation of the framework is
described and analyzed. This paper describes the first clean-slate
architecture aligned with the work done within the ISO/IEC Future
Network working group. |
ETRI Journal, vol.35, no.1, pp.7-17. |
February 2013 | - |
RG | ||
| Enabling SCI-FI: Service-oriented Context-aware and Intelligent Future Internet |
Gonzalez, A. J., Martin de Pozuelo, R., German, M., Alcober, J., Pinyol, F., & Ghafoor, K. Z. |
Internet is becoming a
huge heterogeneous and dynamic network that is growing beyond its
architectural limits. The scaling up of the number of communicating
nodes and services is leading the Internet to an architectural crisis
which in turn makes it difficult to provide services efficiently
considering the requirements and context conditions of users. The
Information-Centric Networking (ICN) approach proposes a network where
the main paradigm is not an end-to-end communication between hosts, as
in the current Internet. Instead, an increasing demand for efficient
distribution of content has motivated the development of architectures
that focus on information objects. ICN supports the proliferation of
services and contents allowing seamless access to them. This work
proposes a context-aware service negotiation protocol which will enable
to find and compose services whilst meeting requesters' requirements
and, consequently, maximizing the QoE of users. We also provide the
main details of a first implementation of the proposed service-oriented
solution (SCI-FI) and discuss the gathered results. |
The 7th International Conference on Future Internet Technologies |
June 2012 | Seoul, Korea |
RG | ||
| In-Network Service Selection and Composition Based on User and Network Context |
Gonzalez, A. J., Martin de Pozuelo, R., Pinyol, F., & Alcober, J. |
The bandwidth demand
growth and a restricted layer structure have led current Internet to an
architectural crisis. Load on the network increases, motivating the
appearance of patches aimed to amend some inherent problems of the
Internet, which were not considered in its inception. Inter-layer
communication in current TCP/IP stack is completely rigid. This
rigidity, and its collateral effects, has not only led to different
cross-layer approaches, but it has also been a factor in the appearance
of sub-layers not considered in the original design, violating the
layered structure of the stack. These practices pose serious
interoperability issues and provide particular solutions to every
problem preventing their reuse. Thus, clean-slate Internet
architectures appear in order to propose novel architectures for the
Future Internet (FI) taking into consideration the limitations of
current TCP/IP and the lessons learned from the past. Furthermore,
network applications and services continuously evolve, increasing its
complexity and requirements, while diverging from the end-to-end
principle. New services and computing paradigms require new modes of
interaction, new features (identification, contextawareness, seamless
service discovery and composition, etc.) and clean solutions to known
open issues (mobility, security, flexibility, etc.). However, while it
is not clear how the current Internet architecture will be able to
handle these new requirements, what seems evident is the need of
introducing strategies in the network that guarantee a certain level of
Quality of Service (QoS) and Quality of Experience (QoE). Additionally,
in the last few years, distributed software systems have become more
dynamic, allowing transparent distribution, self-reconfiguration,
portability, etc. As a consequence, new computational paradigms such as
Pervasive and Ubiquitous Computing or the Internet of the Things have
emerged. |
Euro-NF International Workshop on Traffic and Congestion Control for the Future Internet (pp. 3-4) |
31 March 2011 | Volos, Greece |
GS |
||
| Context-aware multimedia service composition using quality assessment |
Gonzalez, A. J., Martin de Pozuelo, R., Alcober, J., Pinyol, F., & Ghafoor, K. Z. |
With the proliferation of
multimedia capable devices, media services have to deal with
heterogeneous environments where very different types of terminals wish
to receive content anywhere and anytime. This situation motivates the
appearance of multimedia services that adapt contents to the specific
context of users. However, current Internet architecture is based on a
rigid layered model, which makes difficult to introduce new
functionalities efficiently. To solve this, Service Oriented
Architectures (SOA) appear with the goal of proposing new architectures
based on services that can be invoked when and where necessary. This
work introduces how SOA paradigm can be applied to context-aware
multimedia communications. In addition, a scoring function for
selecting different service implementations is presented and
particularized for a case of selecting transcoding functions taking
into account different quality assessment metrics. |
Multimedia and Expo (ICME), 2011 IEEE International Conference on (pp. 1-6). IEEE (toc) |
11-15 July 2011 |
Barcelona |
RG | ||
| Costing framework for service-oriented future internet architectures: empowering requester's choice |
Gonzalez, A. J., Martin de Pozuelo, R., Alcober, J., Pinyol, F., Gutierrez, A., & Monguet, J. M. |
Internet is evolving fast
thanks to the rising of new services and applications. This situation
allowed realizing about current Internet deficiencies. Nowadays,
researchers are proposing novel Internet architectures avoiding the
rigidity of the layered structure posed by the TCP/IP stack. Service
Oriented Architectures (SOA) principles are appropriate for proposing a
flexible and scalable Internet architecture, which relies on the
combination of services according to the requester needs. In this
context it is necessary to introduce a framework for cost and price
that enables requesters and providers to interact and create new
business models for the Future Internet (FI). This work proposes a
novel costing framework for providing services adapted to requester
requirements. |
Proceedings of the 6th International Conference on Future Internet Technologies (pp. 84-90). ACM |
13-15 June 2011 |
Seoul, Korea | RG | ||
| Raudos: Interactive multiplatform audiovisual delivery |
E. Burgoa, M.Rovira, F.
García, P. Arce, J. M. Quinteiro, E. Loizaga, J. Martinez, J.Tejada,
M.Zorrilla, J. Lopez, C.Galicia, F. Pinyol, J.C. Guerri, J. V. Llácer,
N.Martínez, J. Ágreda, A. Arrieta, D.Gonzalez, E. Martel, and V.Beviá |
ap | This paper proposes a new
multi-device and multi-technology audiovisual content delivery system.
Its main features are interactive applications, content and
advertisement recommendation, and virtual character guidance. |
Euro ITV 2010 8th European Conference on Interactive TV and Video (proceedings) |
June 2010 | Tampere, Finland | ||
| Media content management platform |
F. Pinyol Margalef, M. Rovira, J.Borrell, and G. Fernandez Ubiergo |
ap | The Media Content
Management Platform (MCMP) is a wide standard-based media content
cataloguing and managing system which objective is the uploading,
searching and viewing of on-demand videos and TV channels. Currently,
some cataloguing systems do not store enough information about their
contents, and this content data does not follow any metadata standard,
so they do not use an interoperable metadata model. On the contrary, MCMP stores in its database a large list of media content metadata based on standards such as MPEG-7, MPEG-21 and TV-Anytime. It allows having an extensive and complete media content description with a standard and interoperable system. This metadata is structured in core metadata, semantic metadata and technical metadata. Thanks to this approach content customization according to user preferences, better searching system and an extensive and comprehensive content information for the user can be provided. All this capabilities raise the complexity of media content management, but it does not decrease the usability, otherwise it provides to users and administrators more tools and possibilities to manage media content data more efficiently and effectively. The system allows user-generated or professional videos, to be streamed with RTSP, or television channels described by SDP files. |
Euro ITV 2010 8th European Conference on Interactive TV and Video (proceedings) | June 2010 | Tampere, Finland | ||
| Usage of thin clients on stb for secure interactive applications |
F. Pinyol Margalef, M.Rovira Vall, A.Iborra Garcia, G. Fernandez Ubiergo, C. del Ojo Elias, D. Hernando, and T. Felguera |
In our days, interactive
TV has become an important distribution channel for service sector
companies like banks or sellers. In that direction, the use of cloud
computing solutions are today one of the most effective ways to provide
new services and features to end users in an elastic and immediate
manner. We have developed a solution based on open standards, which
uses a cloud computing infrastructure to provide a great level of
security, usability and multi-platform capability. The user connects
through the VNC protocol to a virtual machine server held by the
company, which provides a virtual browser session, which is used to
securely connect to the company website to perform sensible operations.
|
NEM Summit 2010 |
2010 |
Barcelona |
RG | ||
| Frame-accuracy Synchronisation for Mobile TV Interactivity |
Francesc Pinyol Margalef, Alejandro López and Gabriel Fernández |
Interactive applications
for mobile TV are usually related to the audiovisual content, but not
very precisely. In some applications, like object-based interactivity,
where the user can retrieve rich media information associated to moving
objects with just the direct interactivity by the touch screen of the
mobile device, frame-based precision is required. As DVB-H transmits
audiovisual content in MPEG-4 formats, the natural choice for adding
rich content seems to be LASeR (MPEG-4 part 20) scene description. In
this paper we present a mechanism to achieve frame-accuracy
synchronisation between audio, video and LASeR streams. We also
introduce its inclusion to an end-to-end system that implements the
whole chain of creation, transmission and reception of interactive
applications for mobile devices. |
IEEE International Symposium on Broadband Multimedia Systems and Broadcasting (BMSB) 2009 |
13-15 May 2009 | Bilbao |
RG | ||
| Active Objects in Interactive Mobile TV |
J. Deigmöller, G.
Fernàndez, A. Kriechbaum, A. López, B. Mérialdo, H. Neuschmied, F.
Margalef, R. Trichet, P. Wolf, R. Salgado et al. |
pdf |
The porTiVity project is
developing a converged rich media iTV system, which integrates
broadcast and mobile broadband delivery to portables and mobiles and
which will enable the end-user to act on moving objects within TV
programmes. The developments of the project include the playout of
portable rich media iTV and the middleware, data and presentation
engine in the handheld receiver. In this demonstration, we will
present, on the production side, the Live Annotation Tool which allows
the video editor to define and include active objects in Live TV
Programs, and on the user side, the interaction with active objects on
a mobile terminal. |
Proceedings of the 15th International Multimedia Modeling Conference on Advances in Multimedia Modeling. Springer-Verlag Berlin, Heidelberg, pp. 224–226 |
7-9 January 2009 | Sophia-Antipolis, France |
RG |
|
| Portivity: object based interactive mobile TV system |
J. Deigmöller, G.
Fernàndez, A. López, B. Mérialdo, H. Neuschmied, F. Pinyol, R. Trichet,
P. Wolf, R. Salgado, F. Milagaia, S. Glaser, A. Duffy |
The porTiVity project is
developing a converged rich media iTV system, which integrates
broadcast and mobile broadband delivery to portables and mobiles and
which will enable the end-user to act on moving objects within TV
programmes. porTiVity has developed tools which enable producers to
link rich media information with objects in the TV programme. The
developments of the project include the playout of portable rich media
iTV and the middleware, data and presentation engine in the handheld
receiver. For middleware, metadata and the application layer, the
project makes use of international standards such as MPEG-4 A/V, MPEG-
4 LASeR, MPEG-7 Metadata and DVB-H. On the distribution side, the open
standard MXF is used as material exchange format to deliver A/V content
to the authoring suite and to distribute the authored rich media
information between authoring suite and playout centre. In order to
test the overall system and to show its feasibility, two different
services were implemented: »Spur & Partner« and "iSports". |
NEM Summit 2008 Conference Proceedings, pages 146-151, ISBN 978-3-00-025978-4 |
13-15 October 2008 |
Saint Malo, France |
|||
| Intelligent playout for the optimal IP distribution of H264 SVC/MDC services over broadband and broadcast platforms |
Prats, F.E.; Reguant, V.D.; de Pozuelo, R.M.; Margalef, F.P.; Ubiergo, G.F. |
This paper introduces an
approach for the optimal management of a H264 SVC (Scalable Video
Coding)/MDC (Multiple Description Coding) playout. The solution
presented uses optimization and control strategies depending on the
different type of delivered services, the terminals that will consume
these services, the load process of the video servers and the network
conditions. |
Broadband Multimedia Systems and Broadcasting, 2008 IEEE International Symposium on, Page(s):1 - 5, ISBN: 978-1-4244-1648-6 Digital Object Identifier 10.1109/ISBMSB.2008.4536636 |
31 March 2008 - 2 April 2008 | Las Vegas | RG |
||
| Delivery of H264 SVC/MDC streams over wimax and DVB-T networks |
Reguant, V.D.; Prats, F.E.; de Pozuelo, R.M.; Margalef, F.P.; Ubiergo, G.F. |
This paper introduces an
approach for the optimal delivery (encapsulation and signalling) of
video streams coded using H.264 scalable video coding (SVC) combined
with multiple description coding (MDC). The solution presented uses
optimization and control strategies depending on the different type of
delivered services, the terminals that will consume these services, the
load process of the video servers and the network conditions. |
Consumer Electronics, 2008. ISCE 2008. IEEE International Symposium on Page(s):1 - 4, ISBN: 978-1-4244-2422-1 Digital Object Identifier 10.1109/ISCE.2008.4559440 |
14-16 April 2008 | Vilamoura, Portugal | RG | ||
| Adaptive predictive coding of ultrasound images |
C. de Solà Fàbregas, F. Piñol i Margalef |
Ultrasonic imaging has become an
important modality in the field of medical imaging systems, making
necessary to develop an efficient method to compress and store these
images, while retaining the image fidelity. We present a lossless
compression approach which exploits the particular characteristics of
the ultrasound echo-cardiographic instrumentation based on a predictive
adaptive coding method for compression. |
Proc. of the 11th Int. Symposium in Computer Assisted Radiology and Surgery, p. 1004 |
25-28 June 1997 |
Berlin, Germany |
RG | ||
| Applying JPEG compression to a Geometric Transformation of Ultrasound images |
C. de Solà, F. Piñol i Margalef |
A novel approach for compressing
ultrasonic images is presented. In ultrasonic images data are acquired
along radial (polar) sectors. The scan lines, which are arcs, are
represented in the raster image in Cartesian coordinates. Therefore,
even though the image is represented in Cartesian coordinates,
correlation between pixels along certain arcs is higher than between
pixels along horizontal lines. The authors exploit this knowledge to
achieve a higher compression ratio than that possible by applying JPEG
compression to the ultrasonic image. The authors' method transforms the
data into polar coordinates. JPEG compression is applied to this
transformed image |
Proceedings of the 18th Annual International Conference of the IEEE Engineering in Medicine and Biology (EMBS96) |
31 October - 3 November 1996 |
Amsterdam, The Netherlands |
RG | ||
| The Flying Cylinder: a New Algorithm for Filament Recognition in Noisy Stereo Images |
Francesc Piñol i Margalef, Patrick Furrer, Murat Kunt, Jacques Dubochet |
We present a new method of automatic
3D filament representation which uses stereo micrographs to reconstruct
three-dimensional trajectories of filament-like objects as DNA
molecules. The method deals with low contrasted and noisy images, as
obtained from cryovitrified samples by means of electron microscopy.
The three-dimensional information is extracted from skeletizing
simultaneously both images of a given stereo-pair, instead of
processing them separately. The main advantages of the technique are
reproducibility and speed, compared to the reconstruction done by
manual registration, i.e., clicking on the stereo micrographs. |
Journal of Structural Biology,Elsevier, Vol. 116/1, pp. 25-29 |
January-February 1996 |
- |
RG | ||
| Determination of DNA persistence length by cryo-electron microscopy.
Appendix: I. Persistence length of DNA molecules confined to the thin
liquid layer. II. From stereo-micrographs of DNA molecules to their
reconstructed trajectories. Automatic procedure of filament recognition
and its adaptation for the measurement of DNA persistence length |
V. Katritch, J. Bednar, P. Furrer, F. Pinol-i-Margalef, M. Kunt, A. Vologodskii, A. Stasiak, and J. Dubochet |
Axial deflection of DNA
molecules in solution results from thermal motion and intrinsic
curvature related to the DNA sequence. In order to measure directly the
contribution of thermal motion we constructed intrinsically straight
DNA molecules and measured their persistence length by cryo-electron
microscopy. The persistence length of such intrinsically straight DNA
molecules suspended in thin layers of cryo-vitrified solutions is about
80 nm. In order to test our experimental approach, we measured the
apparent persistence length of DNA molecules with natural "random"
sequences. The result of about 45 nm is consistent with the generally
accepted value of the apparent persistence length of natural DNA
sequences. By comparing the apparent persistence length to
intrinsically straight DNA with that of natural DNA, it is possible to
determine both the dynamic and the static contributions to the apparent
persistence length. |
Journal of Molecular Biology, Elsevier, Vol. 254/4, pp. 591-594 |
8 December 1995 |
- |
|||
| 3D Reconstruction of DNA Molecules |
Francesc Piñol i Margalef, Patrick Furrer, Jacques Dubochet |
Pour étudier la forme
tridimensionnelle des molécules d'ADN, on a développé une méthode qui
consiste à vitrifier une fine pellicule de solution observée ensuite
par cryo-microscopie électronique. Le spécimen étant très sensible aux
dommages causés par le faisceau, seules deux images à faible dose de la
même molécule sont enregistrées. Cependant, ces micrographies souffrent
à la fois d'un contraste et d'un rapport signal sur bruit extrêmement
faibles. L'expérience montre que le suivi manuel effectué par un être
humain s'avère assez peu reproductible. Pour améliorer la précision est
la fiabilité des mesures on a développé une approche automatique où
toute la recherche est faite en trois dimensions (3D). Elle imite la
façon dont le cerveau semble procéder en requérant la continuité du
filament et en incluant la flexibilité limitée de l'ADN. En plus, le
fait de travailler en 3D devrait aider à résoudre quelques
incertitudes, comme les croisements et les régions perpendiculaires à
l'axe de tilt. |
Proceedings 15ème Colloque sur le Traitement des Signaux et Images GRETSI, p. 893 |
18-21 September 1995 | Juan-les-Pins, France | RG |
http://www.francescpinyol.cat/projectes.html
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